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Superresolution microscopy enables fluorescence imaging of structures too small for traditional methods, such as deconvolution or confocal microscopy. Carl Zeiss has integrated two methods of superresolution into one, turn-key platform. The ELYRA system can be configured for superresolution structured illumination (SR-SIM) and/or photoactivated localization microscopy (PAL-M). It can also be combined with our laser scanning microscopes, the LSM 780 or LSM 710.
PAL-M provides the highest resolution commercially available and is exclusively integrated into the ELYRA P.1. By sequentially imaging sparse subsets of fluorophores and precisely determining their localization, resolution down to 20 nm in XY and 100 nm in Z is possible. PAL-M works with many photoactivatable and photoconvertible dyes. In addition, many conventional fluorophores, such as Alexa Fluor® 488, Alexa Fluor® 633, Cy3 and Cy5 can be imaged at the same incredible resolution using the closely related localization technique dSTORM. There is a wide variety of fluorescent proteins and antibody-conjugated dyes available for the ELYRA P.1.
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